Pristane‐induced arthritis in mice: V. Susceptibility to pristane‐induced arthritis is determined by the genetic regulation of the T cell repertoire

摘要

AbstractObjectivePristane‐induced arthritis (PIA) is an experimental seropositive arthritis that is characterized by serologic and cellular immune abnormalities and is dependent on the presence of a competent CD4+ T cell population. We examined the regulation of PIA by genes of the major histocompatibility complex (MHC) and theMls‐1loci to determine whether the selection of the T cells that infiltrate arthritic joints is a critical factor in disease susceptibility.MethodsGenetic regulation of PIA was investigated using F1hybrid and congenic strain analysis to determine the influence of MHC andMls‐1genes. The T cell receptor Vβphenotypes of lymph node cells and T cells infiltrating arthritic joints were examined with 2‐color flow cytometry and reverse transcription‐polymerase chain reaction techniques.ResultsF1hybrid offspring from 2 major PIA‐susceptible strains (DBA/1 × BALB/c) were resistant to the induction of arthritis because of the interaction between genes of the MHC and theMls‐1loci, which modified the T cell repertoire. This conclusion was supported by the observed resistance to PIA in BALB/c‐Mls‐1amice, where T cells expressing the Vβ8.1 and Vβ6 phenotypes were absent. The receptor phenotype of T cells infiltrating arthritic joints in DBA/1 mice was markedly skewed toward Vβ8.1 and Vβ6 compared with the population observed in lymph nodes from either PIA or normal control DBA/1 mice.ConclusionThe data support the hypothesis that PIA is a T cell‐mediated disease. While pristane causes a polyclonal T cell expansion that gives rise to lymphadenopathy, the development of arthritis in susceptible strains of mice occurs due to the preservation of specific T cell subsets with the capacity to i