The effect of protection of platelet‐rich plasma against experimental ischemia/reperfusion injury in the rat ovary on in vitro fertilization outcomes

摘要

Abstract Objective Ovarian torsion is a common cause of local ischemic damage, reduced follicular activity and infertility. This study aimed to investigate how well platelet‐rich plasma (PRP) protects against experimental ischemic (I) and ischemia‐reperfusion (I/R) injury in rat ovaries and its effect on in vitro fertilization (IVF) outcomes. Method Fifty‐six adult female Sprague–Dawley albino rats were randomly assigned to six groups of eight animals each: Sham, Ischemia, I/R, Sham + PRP, I?+?PRP, and I/R?+?PRP. The remaining eight animals were used to prepare the PRP. The ischemia groups were subjected to bilateral adnexal torsion for 3?h, while the I/R and I/R?+?PRP groups received subsequent detorsion for 3?h. Intraperitoneal (i.p.) PRP was administered 30?min prior to ischemia (I?+?PRP) or reperfusion (I/R?+?PRP). The ovaries were stimulated through an intraperitoneal injection of 150–300 internal units of IU/kg PMSG. After ovulation induction, oocytes were taken from the ovaries, and IVF was performed. Results The number of MII oocytes reached the highest number with 4.63?±?0.74 in the S group and had the lowest number with 0.50?±?0.53 in the I/R group. There were statistically significant differences for the number of embryos obtained on the second day between the I and I?+?PRP groups and the I/R and I/R?+?PRP groups (p?=?0.000). In comparing anti‐Müllerian hormone 1 (AMH1) and AMH2 values within the group, the highest decrease was observed in the I and I/R groups. Conclusion PRP is effective in minimizing ovarian damage and preserving ovarian reserves following ovarian torsion.