Irradiation Effects on Wound Contraction Using a Connective Tissue Model

摘要

To analyze irradiation effects on wound contraction, fibroblasts harvested from human oral mucosa were Irradiated (single 2-, 4-, 6-, 8-, and 10-Gy exposures of x-rays). The irradiated fibroblasts were examined for growth ability, and they were cultured three-dimensionally in hydrated collagen gels. The irradiation inhibited growth of fibroblasts and contraction of collagen gels in a dose-dependent manner. In addition, inhibition depended on the duration after exposure. The organization of actin filaments of fibroblasts in this model was examined with fluorescent dye-conjugated phalloidin. Many elongated cell projections were formed in cells of the control group, but the cell projections were almost disrupted in the irradiated cells. X-ray irradiation is thus shown to inhibit wound contraction by affecting the organization of actin filaments in fibroblasts.Yanase A, Ueda M, Kaneda T, Toril S, Matsuyama M. Irradiation effects on wound contraction using a connective tissue model. Ann Plast Surg 1993;30:435–440