Gradient elution under hydrostatic equilibrium in liquid chromatography. Part 1

摘要

AbstractThe theory of gradient elution under hydrostatic equilibrium is developed for the case where only one reservoir and one mixing chamber are used and where both solvents have equal densities. Given the shapes of the two vessels, effluent concentration curve equations are deduced for different mixing chamber‐reservoir combinations. On the other hand, given the equation for the effluent concentration curve, the reservoir cross‐sectional area, as a function of height, can be deduced when the mixing chamber has a constant cross‐section. The varying cross‐section of the reservoir is accomplished by inserting thin discs of different areas on top of one another inside a regular vessel of constant cross‐section.Except for complicated gradients, a given gradient can be accomplished using a reservoir with a cross‐sectional area which varies linearly with height. In the case of complex gradients, a reservoir having two or more linear segments becomes necessary. A numerical method is given for the calculation of the continuously varying reservoir cross‐section for the exact duplication of a g