MOLECULAR IDENTIFICATION OF COAT PROTEIN GENE OF MUNGBEAN YELLOW MOSAIC VIRUS (MYMV) IN MACROTYLOMA UNIFLORUM LAM. (VERDC.) WITH METEOROLOGICAL ASPECTS FROM NORTHERN INDIA

摘要

The most destructive yellow mosaic disease is mainly caused by begomovirus and transmitted by whitefly (Bemisia tabaci) affecting legumes production in India. The present investigation was carried out to identify coat protein gene of Mungbean Yellow Mosaic Virus (MYMV) in horsegram accessions showing yellow mosaic symptoms. Disease causing virus was detected by using Polymerase Chain Reaction (PCR) with begomovirus specific degenerate primers. The amplified region of 951bp was sequenced and submitted to GenBank (Accession number MT345791.1). Sequence analysis revealed the highest similarity of 99.16% with Mungbean yellow mosaic virus (Urdbean: New Delhi:2011). A total of 48 accessions of horsegram were screened for average disease incidence and well correlated with meterological parameters. The maximum average disease incidence of yellow mosaic disease was found to be 46.66% (L9), whereas L4, L14, L29 and L30 accessions showed 100% germination of seeds. The average maximum temperature (34.37°C), minimum temperature (24.80°C) and relative humidity (69.72%) were recorded whereas during the experiment, the total rainfall was found to be 111.20mm. It was concluded that the abrupt changes in meteorological parameters cause the progression of MYMV disease incidence under natural field conditions.