Recombinant M13Hol phage containingEcoR1 restriction endonuclease fragments B, E, and F of adenovirus type 2 (Ad2) DNA were constructed by cloning into the uniqueEcoR1 site of the replicative form of the phage M13Hol176 DNA. Polarity of the adenovirus inserts in recombinant molecules was deduced by the following procedures: Viral DNA fragments obtained from Ad2 DNA molecules were purified, denatured, and subjected to electrophoresis. the separated DNA strands were transferred from agarose to nitrocellulose by the Southern procedure and hybridized with radioactive 3′‐end labeledHaeIII fragments of the recombinant phage DNAs. This procedure provided a rapid test for assaying strandedness of the cloned fragme
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机译：通过克隆到噬菌体 M13Hol176 DNA 复制形式的独特 EcoR1 位点，构建含有 2 型腺病毒 （Ad2） DNA 的 EcoR1 限制性核酸内切酶片段 B、E 和 F 的重组 M13Hol 噬菌体。通过以下程序推断重组分子中腺病毒插入片段的极性：从Ad2 DNA分子中获得的病毒DNA片段被纯化，变性并进行电泳。通过Southern程序将分离的DNA链从琼脂糖转移到硝酸纤维素，并与重组噬菌体DNA的放射性3′末端标记的HaeIII片段杂交。该程序为测定克隆的碎片的搁浅性提供了一种快速测试
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