A METHOD FOR ISOLATION OF CHROMOSOMAL AND PLASMID DNA FROM YERSINIA ENTEROCOLITICA FOR SIMULTANEOUS AMPLIFICATION BY POLYMERASE CHAIN REACTION: A POSSIBLE MODEL FOR OTHER BACTERIA

摘要

A commercial DNA isolation kit was evaluated for simultaneous isolation of chromosomal and plasmid DNA from Yersina enterocolitica for polymerase chain reaction amplification. The genomic and plasmid DNA samples obtained by use of the kit were suitable for use in polymerase chain reactions both individually and in multiplex reactions. The results obtained by the use of the kit suggest that this kit may be applied to isolate both genomic and plasmid DNA from other microorganisms for polymerase chain reaction amplification.