AbstractThe metabolism of benzo(a)pyrene [B(a)P] to organic soluble and water soluble metabolites by transformable C3H10T1/2CL8 mouse embryo fibroblasts was studied as a function of time, B(a)P concentration, and cell density. The total formation of organic‐soluble and water‐soluble metabolites increased with incubation time from 4 to 48 h and with B(a)P concentration from 4 to 40 μM. As cell density increased, the metabolic rate decreased for organic‐soluble and water‐soluble products between 6,300 and 54,000 cells/cm2probably due to decreases in B(a)P concentrations to values below saturation. Specific organic‐soluble metabolites identified were B(a)P‐pre‐9, 10‐diols, B(a)P‐9,10‐diol, B(a)P‐7,8‐diol, B(a)P‐3,6‐quinone, B(a)P‐3‐phenol, and B(a)P‐9‐phenol. Water‐soluble metabolites were subjected to enzymatic hydrolysis with β‐glucuronidase and aryl sulfatase to identify specific conjugated products. The sulfate conjugated metabolites identifed were B(a)P‐7,8‐diol, B(a)P‐pre‐9, 10‐diols, B(a)P‐9, 10‐diol, and B(a)P‐3, 6‐quinone. The β‐glucuronic acid metabolites identified were B(a)P‐pre‐9, 10‐diols, B(a)P‐3, 6‐quinone, and B(a)P‐3‐phenol. Patterns of metabolite formation rates are discussed as to their possible effect on morphological transformati
展开▼
