Tumor Necrosis Factor-alpha and IL-17A Activation Induces Pericyte-Mediated Basement Membrane Remodeling in Human Neutrophilic Dermatoses

摘要

Sweet syndrome (SS) is a prototypical neutrophilic dermatosis, a class of inflammatory diseases marked by elevated levels of tumor necrosis factor (TNF)-alpha and IL-17A, pathologic neutrophil recruitment, and microvascular remodeling. Histologic analyses of four matrix proteins collagen I and IV, laminin, and fibronectin in skin biopsies of patients with SS reveal that the basement membrane of dermal post capillary venules undergoes changes in structure and composition. Increased neutrophil recruitment in vivo was associated with increases in collagen IV, decreases in laminin, and varied changes in fibronectin. In vitro studies using TNF-alpha and IL-17A were conducted to dissect basement membrane remodeling. Prolonged dual activation of cultured human pericytes with TNF-a and IL-17A augmented collagen IV production, similar to in vivo remodeling. Co-activation of pericytes with TNF-alpha and IL-17A also elevated fibronectin levels with little direct effect on laminin. However, the expression of fibronectin-and laminin-specific matrix metalloproteinases (MMPs), particularly MMP-3, was significantly up regulated. Interactions between pericytes and neutrophils in culture yielded even higher levels of active MMPs, facilitating fibronectin and laminin degradation, and likely contributing to the varied levels of detectable fibronectin and the decreases in laminin observed in vivo. These data indicate that pericyte-neutrophil interactions play a role in mediating microvascular changes in SS and suggest that targeting MMP-3 may be effective in protecting vascular wall integrity.