Simultaneous Multi-Wavelength UV-Visible Detection for HPLC Using a Diode-Array Spectrophotometer#x2014;A Versatile Software Package

摘要

A BASIC program was developed to monitor absorbance simultaneously at various wavelengths using an HP8451A Diode Array Spectrophotometer linked to a HPLC system and equipped with a flow cell. The program measures a maximum of ten wavelengths and takes a maximum of 240 measurements for each wavelength, using only 16K of memory and a dual disc drive. The program includes the options of replotting using a different ordinate scale and saving the numerical data on disc.