The basis for major histocompatibility complex (MHC) and immunoglobulin gene control of helper T cell idiotopes

摘要

AbstractBALB/c helper T cells, prepared against 2,4,6‐trinitrophenyl (TNP)‐derivatized syngeneic spleen cells, fail to recognize other hapten modifications [(4‐hydroxy‐3‐nitrophenyl)acetyl, fluorescein isothiocyanate] of syngeneic cells, as well as TNP‐derivatized cells from major histocompatibility complex‐congenic donors. T helper cell interactions with “presenting” cells and “target” B lymphocytes are inhibited by monoclonal antibodies directed either to the hapten or to I‐A molecules on target cells. Helper activity is also inhibited by monoclonal anti‐idiotypic antibodies directed to an idiotope expressed by the TNP‐binding myeloma protein MOPC 460, but not by soluble TNP‐protein conjugates. The study of congenic mouse strains revealed that while the fine specificity of TNP recognition and the quantitative levels of M460 idiotope expression are I‐A linked, the expression of the M 460 idiotope by helper cells is controlled by IgCh‐linked genes. Absence of anti‐idiotope inhibition of helper cells prepared in anti‐μ‐suppressed mice suggests, however, that immunoglobulin idiotype expression by T cells results from network interactions selecting available lymphocyte repertoires wh