Supplementation of Seminal Plasma-Heparin Binding Proteins to Capacitation Medium Increases In Vitro Acrosome ReactionPercentage in Beetal Bucks

摘要

This study was focused evaluating the effect of heparin binding proteins (HBP) on in vitro capacitation and acrosome reaction (AR) in Beetalbucks. HBP were purified from pooled seminal plasma of six bucks to evaluate their effect on in vitro capacitation. Semen, washed twice with TALP medium was incubated in TALP, supplemented with glucose, BSA, heparin and HBP for 6 hrs at 37°. A control was also run without HBP. Incubated samples were evaluated for motility, viability, acrosome reaction and innermitochondrial membrane potential (IMMP) after every 2 hrs. Standardization of HBP concentration on in vitro acrosome reaction revealed 150 pg/ml HBP as an optimum dose. Based on sperm parameters, six bucks were divided into two groups i.e. GT (> 80% motility) and G-II (0.05) increase in LPO during capacitation and AR was also observed. This study concluded thatSP-HBP enhanced the rate of in vitro AR in beetal bucks.