Use of fluorescence spectroscopy for diagnosis of hypoxia and inflammatory processes in tissue

摘要

In vivo laser-induced fluorescence spectroscopy (LIFS) is primarily used in oncology for the diagnosis of malignant tumors. This paper provides background for and describes experiments modeling nonmalignant local hypoxia and inflammation. LIFS techniques were used to assess the dynamics of induced fluorescence from endogenous porphyrins in the first case and the Photosens exogenous photosensitizer in the second case. In both cases, the fluorescence intensity was observed to be higher in the pathological area than in an intact area. This provides a strong impetus for taking a second look at the use of LIFS in oncology and also provides the foundation for a promising in vivo diagnosis method for ischemic hypoxia and inflammatory processes in areas other than oncology. (C) 2016 Optical Society of America.