We demonstrate the nanosecond time-gated spectroscopy of plume in laser ablation of biological tissue. Clear and sharp peaks of calcium ion (Ca{sup}+) appear in the spectrum although the Ca content is only 0.1% in human hair and nail. A preliminary experiment was made toward a possible diagnosis, where human hair and rat nail were tested to detect the Ca content. This specific spectroscopy is potential for novel diagnoses including monitor of daily intake of Ca and a screening diagnosis of osteoporosis.
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