Abstract.One liter of strongly Australia antigen positive plasma was subjected to a modified Cohn fractionation and samples from the various steps of the process were assayed quantitatively for Australia antigen. More than 85% of the original quantity of Australia antigen was recovered from fraction IV. Intermediate amounts, about 2%, were found in fraction III and in the washing fluids after the fibrinogen wash. The final fibrinogen product had no detectable Australia antigen. About 1 % of the antigen was recovered from the albumin. γ‐Globulin before treatment with DEAE‐Sephadex had a low content, about 0.1% of the original amount of antigen, whereas DEAE‐Sephadex treated material (final gammaglobulin) was free of demonstrable Au‐antigen.The findings are discussed in relation to clinical reports on hepatitis risks connected with the various
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