Design,Synthesis and Anticancer Activity of New Benzofuran-Chalcone Hybrids and Their Water Soluble Sodium Salts

摘要

In this study,firstly,1-(7-ethoxy-1-benzofuran-2-yl)ethanone and 1,1’-(7-ethoxy-1-benzofuran-2,4-diyl)diethanone were synthesized for the starting reagent purposes.The synthesized benzofuran-chalcone salts were soluble in water at room temperature.Structural analysis of the synthesized compounds was characterized by elemental analysis,FT-IR and NMR spectroscopy techniques.The anticancer activities of the compounds were determined by SRB viability assay in human lung cancer(A549,H1299)and breast cancer(MCF-7,MDA-MB-231)cell lines.Findings for apoptosis were determined by flow cytometry analysis and the PARP-ELISA method.The results of the in vitro SRB analysis of the compounds showed that some of the chalcone hybrids were very effective on both types of cancer in a dose and time-dependent manner.Treatment of all cancer cell types with these hybrids resulted in a significant increase in the percentage of early and mainly late apoptotic cells,demonstrating their apoptosis-inducing effects via the Caspase 3/7 Activity.