Preparation and Characterization of 5-Fluorouracil Loaded Cellulose and Exosome Nanocarriers: Sustained Release Behavior and Cytotoxicity Studies**

摘要

In recent years, much effort has been devoted to designing nanoparticle drug delivery systems and improving the efficacy of anticancer drugs. This study focused on the preparation of a novel biocompatible nanocarrier based on bacterial cellulose (BC) in the presence of exosome (Exo) for the controlled release of 5-fluorouracil (5-FU), known as 5-FU.Exo@BC. The physicochemical properties of 5-FU.Exo@BC were thoroughly characterized using field emission scanning electron microscopy (FESEM), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). These results confirmed the successful preparation of 5- FU.Exo@BC. The release behavior of 5-FU.Exo@BC compared with 5-FU and 5-FU@BC demonstrated a significant sustained release over 162 h. The release mechanism of the above three systems followed Korsmeyer-Peppas with non-Fickian diffusion for 5-FU@BC and 5-FU.Exo@BC. Furthermore, the viability of HT-29 cells (human colon cancer cell lines) against BC, 5-FU@BC and 5-FU.Exo@BC indicated that 5-FU has promising efficacy in 5-FU.Exo@BC system. Subsequently, the prepared bio-nanocomposite could be suggested as a potential drug delivery system with an effective controlled-release function.