Micropropagation technique is used for rapid seed multiplication in sugarcane. The axillary shoot pathway is considered to be the safest route for the production of true copies from the mother plant. An efficient protocol for micropropagation of sugarcane from shoot tip explants has been described. Shoot tip explants (1.0-1.5cm in length) were inoculated on agar gelled (8.0 g/1) MS media containing BAP and Kn (0.5mg/l each).Numerous shoot were developed within a month, the culture established is thentransferred to the multiplication medium supplemented with BAP, Kn and NAA. Well-elongated and healthy shoots were transferred to half strength MS rooting medium containing 5mg/l NAA and 50g/l sucrose. Several roots were developed from the base of shoots within 12-15 days. Plantlets separated, were kept in bottle containing tap water for the development of secondary roots and then planted in small polythene bags containing soil mixture (soil:sand:compost;l:l;l) for 30 least days in a green house. The plantlets were then shifted to shade house for 2-3 weeks. After hardening, the plantlets were field transplanted at 60cm distance in rows 90cm apart.
展开▼
机译：微体繁殖技术是用于快速种子在甘蔗乘法。途径被认为是最安全的路线真实的副本从母亲的生产工厂。甘蔗的茎尖外植体描述。长度)接种在琼脂稠化(8.0 g / 1)媒体包含BAP和Kn女士(0.5 mg / l每个)。月,是thentransferred建立的文化乘法中补充软面包卷,Kn和乙酰天冬氨酸。女士被转移到一半的力量生根培养基含有5 mg / l NAA和50 g / l蔗糖。在12 - 15天内拍摄基地。分开,被保存在瓶中水二次发展的根源然后在小聚乙烯袋包含种植土壤混合物(土壤:沙子:堆肥;l: l, l)为30至少在一个绿色的房子。然后转移到阴凉的房子的时间为2 - 3周。硬化,苗木移植在60厘米距离行90厘米。
展开▼
