Valorisation of agroindustrial-residues for production of a potent thrombolytic protease from Aspergillus terreus SH72

摘要

Abstract Cardiovascular diseases (CVDs) are the principle cause of indisposition and global fatality. Under certain pathophysiological conditions, formation of fibrin clot causes obstruction in normal blood flow and leads to thrombosis. Excessive treatment cost and side effects of the available thromobolytics prompt the researchers to investigate potentially superior, economic and more acceptable therapeutics for thrombosis. Current study reports cost-effective production of an efficacious fibrinolytic protease (fibpro) from a newly isolated fungal strain Aspergillus terreus SH72 using low-cost agricultural residues as substrates. The fibpro showed fairly good substrate specificity towards fibrin. Molasses as carbon source supported maximum yield of fibpro (168.23 U/mL), however, substantial enzyme titre was produced on other agro-residues i.e. malt (151.15 U/mL), wheat bran (147.78 U/mL), banana leaves (145.20 U/mL), corncob (138.86 U/mL), bamboo leaves (138.07 U/mL), and rice husk (134.10 U/mL) which was higher than that in control i.e. glucose as carbon source (126.77 U/mL). Similarly, several agro-based nitrogen sources were utilized by A. terreus SH72 for producing adequate fibpro yield i.e. sesame cake (196.32 U/mL), spent grains (194.34 U/mL), coconut seed cake (193.95 U/mL), soyameal (193.15 U/mL), malt extract, (191.96 U/mL), ground nut cake (184.83 U/mL), and others. Most significant process variables (molasses, sesame cake, KH2PO4, and incubation time) earmarked based on Plackett–Burman designed experiments were optimized by design of experiment (DoE) based on response surface methodology to attain yield enhancement of 2.03-fold.