Typing of Staphylococcus aureus Obtained from Mastitic Milk of Cattle and Buffalo on the Basis of Coagulase (coa) Gene RFLP Patterns

摘要

A typing procedure based on polymorphism of the coagulase gene (coa) was used to discriminate 32 Staphylococcus aureus isolates obtained from cattle (n= 16) and buffalos' (n= 16) mastitic milk. All the isolates showed coagulase production on plasma obtained from various species of animals and human. Amplification of coa PCR products (400bp, 510bp, 600bp and 650bp) were produced from cattle isolates and five different products (400bp, 510bp, 600bp, 650bp and 680bp) from buffalo isolates. From four coagulase types in cattle isolates five restriction fragment length polymorphism (RFLP) patterns were obtained and from five coagulase types in buffalo isolates six RFLP patterns were obtained. The coa gene amplicon of 600 bp was produced by the maximum number of isolates.