Preparation and Application of Electrochemiluminescence Sensor by Immobilizing Tris(2, 2'-bipyridine) ruthenium(II) on the Surface of Gold Electrode with Silica sol/Nano-Au/PVP/L-cysteine

摘要

In order to establish a simple, sensitive and rapid method for the determination of azithromycin, the gold electrode modified with Silica sol/Nano-Au/PVP/(Ru(bpy)_3)~(2+) was prepared, and the electrochemiluminescence (ECL) method was developed for the determination of azithromycin based on the sensitizing effect of azithromycin on (Ru(bpy)_3)~(2+). Moreover, the electrochemical behaviors and the electrochemical luminescence behaviors of the (Ru(bpy)_3)~(2+) system and the (Ru(bpy)_3)~(2+)-azithromycin system were investigated by ECL respectively. The results show that the proposed electrode showed very good electrochemical activity and electrochemiluminescence response to the (Ru(bpy)_3)~(2+) azithromycin system, under the optimum experimental condition, the linearity of azithromycin concentration to ECL intensity was achieved in the concentration of azithromycin from 1.0 × 10~(-7) to 1.0 × 10_4molL~(-1) and the detection limit (S/N = 3) was 1.33 × 10~(-9)molL~(-1). Through the parallel determination of, 1.0 × 10~(-5) mol~(-1) azithromycin for 8 times, the RSD of results was 1.82%, and the recovery ranging from 97.9% to 101.6% was obtained in the detection of azithromycin tablets and the RSD was 1.36% (n = 5). The proposed method showed high selectivity and sensitivity and simplicity, and it could be applied to the analysis of azithromycin with satisfactory results.