HAPLOTYPE VARIATION OF ESTROGEN RECEPTOR-a (ER-a) GENE EXON 4 IN TURKISH SHEEP BREEDS

摘要

Estrogen receptor a (ERa) gene has previously been found to related with sexual development and reproduction. In this study, on the basis of the sequences of human, cattle and caprine estrogen receptor a (ERa) genes, available in the GenBank database,sets of PCR primers were designed and used to amplify the ovine ERa gene exon 4 region. We identified six single nucleotide polymorphisms (SNP) in the ERa exon 4. Some variations determined for exon 4 g.43A > G, p.T43A; g.49C > T, p.L49F; g. 178A > T, p.T 178S led to changes in the amino acids, but no amino acid changes were determined in g.l8G > C, g.27C > T, g.96G > A. These fragments were deposited in the GenBank database under accession number: JF262030—JF262035. It was noted in particular that White Karaman and Awassi breeds were similar to each other, whereas the Chios breed had a different variation.