BACKGROUND AND OBJECTIVES: A previous study indicated that the extension of whole blood (WB) storage from 8 to 24 h at 20-24 degrees C before the processing of platelet-rich plasma (PRP)-depleted red blood cell (RBC) units had a negative effect on the efficacy of leucoreduction filters. In this study, we further characterized the phenomenon and tested the leucoreduction capacity of two newly developed filters. MATERIALS AND METHODS: Whole blood was stored at 20-24 degrees C and processed at 4-h intervals between 8 and 24 h postcollection. Components were leucoreduced before storage. Efficacy of novel filters to leucoreduce 24-h-hold PRP-depleted RBC units was also evaluated. RESULTS: Using a conventional filter, the mean residual white blood cell (WBC) counts in leucoreduced PRP-depleted RBCs were comparable in units prepared within 12 h from collection but gradually increased upon extended preprocessing storage from 0.36 +/- 0.03 at 12 h to 0.46 +/- 0.21, 0.76 +/- 0.54 and 1.72 +/- 1.76 x 10(6) per unit at 16, 20 and 24 h, respectively. However, the mean residual WBC content in 24-h-hold RBCs was reduced to 0.60 +/- 0.39 x 10(6) and 0.46 +/- 0.13 x 10(6) per units using RC2D and the prototypes B-1582 rev B filters, respectively. CONCLUSION: For PRP-depleted RBC units, the extension of the WB room temperature storage from 8 to 24 h before processing is likely to require the introduction of newly developed filters having an increased leucoreduction capacity in order to meet the maximal residual WBC guideline in the RBCs.
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