Identification and sequence analysis of TLR2 gene in Murrah buffalo.

摘要

Toll-like receptor 2 (TLR2) the most promiscuous of all the TLRs, has been implicated in signaling induced by gram-positive cell walls, peptidoglycan and mycobacterial factors, GPI anchors (Trypanosoma cruzi), lipoarabinomann (Mycobacterium tuberculosis), porins (Nesseria meningitides) and zymosan (yeast cell wall component) and a number of endogenous ligands viz., necrotic cells and their protein byproducts and heat shock proteins (HSP60, HSP 70 and GP96). The present study has characterized TLR2 gene in Murrah buffalo (Bubalus bubalis). A 1152bp partial coding sequence of TLR2 gene was amplified, cloned, sequenced and characterized. The sequence was submitted to Genbank with accession number GU441859. The sequence shared 98% identity with that of Capra hircus, 97% with Bos taurus, Bos indicus and Bison bison, 96% with Boselaphus tragocamelus, 94% with Ovis aries, 82% with Sus scrofa and Eqqus cabalus, and 81% with Canis lupus familaris. The deduced protein sequence showed 95% identity with Bison bison, 94% identity with Bos indicus and Bos taurus, 93% withBoselaphus tragocamelus, 90% with Ovis aries, 89% withCapra hircus, 73% with Sus scrofa, 71% with Equus caballus and Canis lupus familaris and 44% with Gallus gallus. Phylogenetic analysis of the deduced amino acid sequences of various species, revealed that buffalo clustered together with other bovids and Capra hircus, and away from Equus cabalus, Sus scrofa and Gallus gallus depicting the genetic relatedness and divergence among various species. Analysis of the number of synonymous substitutions per synonymous site and non synonymous substitutions per non synonymous site indicated that the nucleotide sequences coding for the TLR2 gene were not under positive selection and null hypothesis of strict-neutrality was accepted.