Cloning and expression of chicken IgG lambda chain in E.Coli.

摘要

Abad Shapouri MRS, Yektaseresht A, Ekhtelat M., Cloning and expression of chicken IgG lambda chain in E. coli, Onl J Vet Res., 19(11): 750-754, 2015. RNA from spleen cells was reverse transcribed into cDNA and amplified, cloned and expressed in E. coli DH5a for production of chicken IgG lambda chain antibody. Expressed protein was analyzed by SDS-PAGE confirmed by Western blotting. Results indicated that the constant region of chicken IgG lambda chain was highly expressed in E. coli as a protein withmass of 53.5 kDa. Reactivity analysis by western blotting revealed high binding affinity of the recombinant protein to conjugated anti chicken IgG.