A novel class of potential prion drugs: preliminary in vitro and in vivo data for multilayer coated gold nanoparticles

摘要

Gold nanoparticles coated with oppositely charged polyelectrolytes, such as polyallylamine hydrochloride and polystyrenesulfonate, were examined for potential inhibition of prion protein aggregation and prion (PrP~(Sc)) conversion and replication, different coatings, finishing with a positive or negative layer, were tested, and different numbers of layers were investigated for their ability to interact and reduce the accumulation of PrP~(Sc) in scrapie prion infected ScGT1 and ScN2a cells. The particles efficiently hampered the accumulation of PrP~(Sc) in ScN2a cells and showed curing effects on ScGT1 cells with a nanoparticle concentration in the picomolar range. Finally, incpbation periods of prion-infected mice treated with nanomolar concentrations of gold nanoparticles were significantly longer compared to untreated controls.