Optimization of Extraction Techniques and Quantification of Amarogentin by Using RP-UFLC Method from Different Swertia Species

摘要

Swertia is used in Indian Ayurvedic herbal systems and in Tibetan folk medicines to cure fever. In this investigation, strategies have been developed to optimize an efficient method for the extraction and quantification of amarogentin from different Swertia species collected from Himalayan and Western Ghats regions of India. Diverse extraction methods such as static extraction (SE), continuous shaking extraction (CSE), and ultrasonic extraction (USE) were evaluated for increased recovery percentage of amarogentin. The results revealed that SE was the more efficient method for extraction of amarogentin from different Swertia species. Extraction in SE at 24 hrs gave the maximum recovery of amarogentin in S. chirayita (5.79 mg/g) followed by S. corymbosa, S. minor and S. bimaculata. S. corymbosa (1.86 mg/g). S. minor with 1.44 mg/g amarogentin was remarkably the best match for S. chirayita among the Swertia species investigated, suggesting best alternatives for chirayita form the Western Ghats.