乳癌における glycogen synthase kinase (GSK)3βの発現の意義

摘要

Aim: This study investigated the association between glycogen synthase kinase 3 beta (GSK3β) and breast carcinoma by focusing on the epithelial-mesenchymal transition (EMT). Method: Immunohistochemical staining of GSK3β and Twist (EMT marker) was performed in 116 cases of invasive breast carcinoma. Immunopositivity was defined as either diffuse (D+) or focal (F+) depending on the size of the immunopositive area. Furthermore, periphery positive cases (P+) were defined by the difference of 10% or more between the immunopositive rates of the periphery and central areas of each case. Double immunochemistry for GSK3p and Twist was performed to examine the coexpression of those. Result: Regarding GSK3β, P+ cases were found to possess more lymphatic vessel invasion than P- cases. In addition, the F+ group had a significantly larger invasive diameter than the D+ group, and many cases showing lymphatic invasion, vascular invasion, lymph node metastasis, and more number of P+ cases (p = 0.004, p = 0.026, p = 0.015, p < 0.001, respectively) were noted. Twist positive cases had a significantly larger invasive diameter than the Twist negative group (p = 0.047). Furthermore, there were many cases showing coexpression of GSK3β and Twist in a group with matching peripheral tendencies between GSK3[3 F+ and Twist, and the invasive diameter was significantly larger than that of the other group (p = 0.019). Conclusion: GSK3p expression of the peripheral area of a tumor can accelerate tumor infiltration and metastasis with respect to EMT. [Original]