Cannabis sativa is comprised of hundreds of individual compounds that can be classified in many chemical families,such as terpenes,amino acids,fatty acids,hydrocarbons,flavonoids,sugars and cannabinoids [1,2].Cannabinoids represents a class of chemicals that are classified as terpenophenolic compounds.There are about 70 terpenophenolic compounds in the cannabinoid class.These are only found in cannabis plants [3].Of the 70 cannabinoids found in Cannabis there are several cannabinoids that are of human physiological and medicinal interest [4].These include the psychoactive A-9-tetrahydrocannabinol(THC),non-psychoactive cannabidiol(CBD)and the non-psychoactive tetrahydrocannabivarin(THCV)[5-8].THC,THCV and CBD are neutral forms of cannabinoids,obtained after a non-enzymatic decarboxylation of delta 9-tetrahydrocannabinolic acid(THCA),tetrahydrocannabivarinic acid(THCVA)and cannabidiolic acid(CBDA).It is the focus of this manuscript to utilise SFC chromatographic stationary phases that have been specifically developed for the isolation and purification of THCA,CBDA,THC,CBD and THCV.These specific cannabinoids require the use of several different stationary phases for optimised separation and purification of them individually.
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