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Spermatozoa survival and fertility of Nera black breeder semen extended with carrot juice

机译:NERA黑色育种精液的生存和育种与胡萝卜汁扩展的生育能力

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摘要

This experiment was conducted to examine the fertilising ability of layer breeder cock spermatozoa extended with normal saline fortified with varying levels of carrot juice using 10 breeder Bovan Nera cocks. Semen was collected from the cocks, pooledand divided into six portions (treatments). Treatment 1(TUS) was undiluted semen which served as a control, while Treatments 2 (TNS) and 3 (TMRS) were diluted at 1:1 with normal saline and modified ringer solution, respectively. Treatments 4 (T25%CJ), 5(T50%CJ), and 6 (T75%CJ) were diluted at 1:1 with normal saline fortified with 25%, 50% and 75% carrot juice, respectively, and stored at room temperature (27.0±0.2 ). Sperm motility and liveability reduced (p<0.05) as time of storage increased. in vivostudy was carried out with 70 hens randomly allocated and inseminated with each of the 5 treatments. The fertility among the treatments was 75% (TNS), 100% (T2), 60% (T3), and 83.33% (T4), while optimum of 60% at day 3 and 50% at day 7 were obtained forT3 and T5 respectively. Duration of fertile period was prolonged by carrot juice for T50%CJ and T75%CJ respectively by 2 and 3 days relative to TNS and 5-6 days for TMRS. Embryonic mortality and hatchability were similar among the treatments.
机译:进行了这项实验,以检查层育种公鸡精子的施肥能力,并使用10种饲养员的Boilder bovan nera公鸡延伸了正常的盐水,并具有不同水平的胡萝卜汁。从公鸡中收集精液,并分为六部分(治疗)。处理1(TU)是未稀释的精液,用作对照,而处理2(TNS)和3(TMR)分别在1:1的情况下用正常的盐水和改性铃声稀释。治疗4(T25%CJ),5(T50%CJ)和6(T75%CJ)在1:1下稀释,正常盐水加强了25%,50%和75%胡萝卜汁,并存储在房间中温度(27.0±0.2)。随着存储时间的增加,精子运动和宜居性降低(p <0.05)。在50种处理中随机分配了70个母鸡,并通过5种治疗中的每一种进行了体内。治疗中的生育能力为75%(TNS),100%(T2),60%(T3)和83.33%(T4),而第3天的最佳最佳为60%,在第7天获得了50%分别。相对于TNS,TNS和3天分别用胡萝卜汁和T75%CJ延长了肥沃期的持续时间,而TMR则为5-6天。胚胎死亡率和孵化性在治疗中相似。

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