High frequency plant production via shoot organogenesis in Leucosceptrum canum Smith (Lamiaceae)

摘要

Maity S. K. & Kundu A. K. 2013. High frequency plant production via shoot organogenesis in Leucosceptrum canum Smith (Lamiaceae). Geophytology 42(2): 139 - 145. Leucosceptrum canum Smith, the only short tree species of Lamiaceae, does not produce viable seeds and is mainly propagated vegetatively. A rapid in-vitro propagation of whole plant was achieved from axillary shoot culture in Murashige and Skoog Medium (MS) supplemented with 6-benzylaminopurine (BAP, 2 mg/1), Adenine sulphate (AdS, 40 mg/1) and thiamin HCl (4 mg/1). The multiplication rate of the explant was 90% and each explant developed 10.53± 0.5 shoots averaging 4.82± 0.3 cm length within 40 days of culture. The regenerated shoots were rooted on MS medium containing indolebutyric acid (IBA, 1 mg/1) and the explants producing 12.53± 0.3 roots per shoot within a period of 30 days. It is direct and more efficient method for ensuring trueness to type than any other in-vitro procedure. The explants were acclimatized by transferring them to earthen pots containing a mixture of autoclaved soil: sand:compost (1:1:1) and they were immediately covered with transparent polybag. This system provides high fidelity micropropagation system for efficient and rapid production of this important plant.