Selective Sensing of Ammonium Ion Over Other Biologically Important Ammonia Derivatives by a Coumarin-Based e-Amino Ester

摘要

Ammonium ions are a waste product of the amino acids metabolism of animals and finally converted to urea in liver. Specific sensing of ammonium ion may helpful for diagnosis of liver malfunction. An efficient coumarin-based e-amino ester has been synthesized and the specific sensing of ammonium ion has been investigated. From single crystal X-ray diffraction analysis, the amide group is out of the plane of the central aromatic core of the compound but the ester group is coplanar and the compound self-assembled to form supramolecular duplex-like structure. The coumarin-based eamino ester successfully discriminates ammonium ion over other biologically important ammonia derivative such as Dglucosamine, ATP, glycine, L-glutamic acid, L-aspertic acid and urea. The coumarin-based e-amino ester shows excellent selectivity as turn-off optical sensor for ammonium ion. Further the biocompatibility of the coumarin-based e-amino ester was studied by DNA binding assay and cellular uptake by mouse macrophage cell line RAW264.7.