Polyphenolic Compound from Brown Macroalga Padina tetrastromatica Imparts Oxidative Stress Tolerance in SH-SY5Y, RAW 264.7, HeLa Cell Lines and in Caenorhabditis elegans

摘要

Marine brown macroalgae are known for producing potent bioactive secondary metabolites such as phlorotannins. Phlor- otannins are the phenolic compounds which can inhibit the oxidative stress via scavenging the free radicals. In this study, phlorotannin from Padina tetrastromatica brown macroalga was collected from Mandapam, Rameswaram, Tamil Nadu and identified by microscopic observation. The compound was extracted and purified by using column chromatography, thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The purified compound showed prominent inhibition of free radicals via reactive oxygen species (ROS) activity in vitro. Further, the compound showed the inhibitory effect of ROS in RAW 264.7 murine macrophage cell line, HeLa human cervical cancer cell line, and SH-SY5Y human neuroblastoma cell line. Further, the effect of the compound on neurotoxicity was carried out in Caenorhabditis elegans as an animal model. The stress-responsive genes daf-16 and sod-1 gene expression studies were performed and results revealed that the aforementioned genes had shown up-regulation in compound treated with acrylamide induced worms. Thus, this compound may prospect for therapeutic applications against the oxidative stress-related complications.