Fluorescence In Situ Hybridization of 16S rRNA in Escherichia coli Using Multiple Photo-Cross-Linkable Probes

摘要

RNA forms various secondary structures depending on its sequence in cells;the influence of secondary structures must be considered when detecting,regulating,or manipulating RNA.In this study,we utilized the cooperativity of multiple photo-cross-linkable assistant probes to evaluate photo-crosslinking for RNAs with complex secondary structures.We succeeded in increasing the fluorescence intensity 39.9 times in the region where detection was difficult owing to the formation of a complex secondary structure in the conventional fluorescence in situ hybridization method.This method enables the detection,regulation,and manipulation of RNA that forms various structures depending on the sequence.