Antioxidant, Antibacterial and Anticancer Performance of Reduced Graphene Oxide Prepared via Green Tea Extract Assisted Biosynthesis

摘要

Green tea extract (GTE) contains polyphenols and can play as a bioreducing and stabilizing agent. This paper describes envi-ronmentally friendly approach for synthesis of reduced gra-phene oxide using GTE (GTE-rGO). The results have confirmed that biomolecules present in green tea extract not only act as bioreductant but also functionalize the surface of rGO due to hydrogen binding and π-π stacking interactions. The 100 ppm of rGTE-rGO showed 0.68 ppm phenols and 0.24 ppm flavones. The rGTE-rGO was found to have highest% of 2,2-diphenyl-1-picrylhydrazyl (DPPH, 73.83%) scavenging activity. Antibacterial activity of GTE-rGO exhibited strong activity against Escherichia coli and Staphylococus. A cytotoxicity evaluation of GO and GTE-rGO was carried out on fibroblast, MCF7 breast cancer and PC3 prostat cancer cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) results showed high viabil-ity loss and toxicity of GTE-rGO against cells especially for PC3 cells. Thus, GTE-rGO because of biomolecules present could potentially act as antioxidant, anticancer and antibacterial agent.