Monolithic macroporous composites constructed by poly (glycidyl methacrylate-2-hydroxyethyl methacrylate)/SiO2 nano-film (P(GMA-HEMA)/SiO2) were prepared and then used as the supports for immobilization of Pseudomonas fluorescens lipase (PFL). The as-prepared macroporous materials were characterized by scanning electron microscope (SEM), N2 adsorption-desorption experiments, Fourier transform infrared (FT-IR) spectroscopy and thermogravimetric (TG) analysis. The immobilization conditions and the properties of the immobilized PFL were investigated. The characterization results revealed that the macroporous SiO2 has a 3D continuous pass-through macropore structure, large pore volume and high specific surface area. After modification with P(GMA-HEMA) and the subsequent immobilization of PFL, the structural characteristics were preserved. Under the optimum conditions, the activity of the immobilized PFL reached to 3773 Ug~(-1) and enzyme activity recovery achieved to 232%. Compared with free lipase, the thermal and pH stabilities of the immobilized lipase were significantly enhanced, and it also exhibited an outstanding reusability.
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