Anti-Inflammatory,Antiallergic and COVID-19 Main Protease(M~(pro))Inhibitory Activities of Butenolides from a Marine-Derived Fungus Aspergillus costaricaensis

摘要

Amid the current COVID-19 pandemic,the emergence of several variants in a relatively high mutation rate(twice per month)strengthened the importance of finding out a chemical entity that can be potential for developing an effective medicine.In this study,we explored ethyl acetate(EtOAc)extract of a marine-derived fungus Aspergillus cosatricaensis afforded three butenolide derivatives,butyrolactones I,VI and V(1-3),two naphtho-γ-pyrones,TMC-256 A1(4)and rubrofusarin B(5)and methyl p-hydroxyphenyl acetate(6).Structure identification was unambiguously determined based on exhaustive spectral analyses including 1D/2DNMR and mass spectrometry.The isolated compounds(1-6)were assessed for their in vitro anti-inflammatory,antiallergic,elastase inhibitory activities and in silico SARS-CoV-2 main protease(M~(pro)).Results exhibited that only butenolides(1 and 2)revealed potent activities similar to or more than reference drugs unlike butyrolactone V(3)suggesting them as plausible chemical entities for developing lead molecules.