Preparation and evaluation of magnetic core-shell mesoporous molecularly imprinted polymers for selective adsorption of amitriptyline in biological samples

摘要

In this study, preparation of magnetic surface molecular imprinted polymer (MMIP) is employed for sensitive solid phase extraction and measurement of amitriptyline (AMT) by spectrophotometric technique. After polymerization, cavities in the polymer particles corresponding to the AMT were created by leaching the polymer by an appropriate solution. The synthesized nanoparticles of magnetic surface molecular imprinted polymer mesoparticles (Fe3O4@SiO2-MIP) were characterized by transfer electron microscopy (TEM), vibrating sample magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), X-Ray Diffraction (XRD) and Brunauer-Emmett-Teller (BET). Also, the pH of zero-point charge (pH(zpc)) for Fe3O4@SiO2-MIP was determined with the pH drift method. The influence of various parameters such as pH, amount of polymer and contact time were investigated by an optimization technique named Box-Behnken design (BBD) and the proposed quadratic model was fitted very well with the experimental data. The validity of optimum parameters and the equation of parameters were investigated by analysis of variance (ANOVA). Also Scatchard isotherm was explored for the equilibrium adsorption investigation and finally, the prepared polymer was successfully applied to the selective identification and the determination of AMT in plasma samples. (C) 2018 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.