Gold nanoparticles for Tc-99m-doxorubicin delivery: formulation, in vitro characterization, comparative studies in vivo stability and biodistribution

摘要

The [Tc-99m]Tc-Doxorubicin-Gold Nanoparticles (Tc-99m-Dox-AuNPs) was formulated as a nanoradiopharmaceutical by different loading procedures to enhance tumor targeting. The formula F1 that was prepared by direct loading of pre-prepared [Tc-99m]Tc-Doxorubicin showed a reasonable in-vitro characterization values, high entrapment efficiency (92 +/- 0.72%), acceptable in vitro release data and convenient in-vitro serum stability up to 24 h. F1 presented high tumor uptake (54%ID/g) with high Target/ Non Target (T/NT) ( approximate to 77) and Drug Target Efficiency percent (%DTE) above 100% at 0.5 h via intra-tumoral injection that prove the increasing of tumor targeting and in-vivo stability by direct loading for pre-prepared [Tc-99m]Tc-Doxorubicin.