Detection of Pathogen Induced Protein in Tea Leaves and Biochemical Changes in Defense Enzymes Following Inoculation withGlomerella cingulata

摘要

Among 18 tea varieties tested for screening resistance against Glomerella cingulata using whole plant inoculation technique, TV-30 was found to be highly resistant while CP-1 was moderately susceptible and TV-22 was most susceptible. Scanning ElectronMicroscopic observations of adaxial and abaxial surface of brown blight infected tea leaves have been studied. Serological assays using PTA-ELISA, Dot immunobinding assay and western blot formats of antigens of healthy and artificially inoculated leaf with G. cingulata of three tea varieties ( TV-30, TV-22 and CP-1) were performed using polyclonal antibody raised against G. cingulata. Pathogen induced protein (24Kda) was detected in susceptible varieties (TV-22 and CP-1) in western blot analysis. Timecourse accumulation of phenylalanine ammonia lyase, tyrosine ammonia lyase, peroxidase and polyphenol oxidase after 24,48 and 72h of inoculation of tea varieties with G. cingulata indicated enhanced enzyme activities in resistant variety (TV-30) in comparision with other susceptible varieties tested. Isozymes of peroxidase and polyphenoloxidase were also characterized.