Response surface methodology based on central composite design for optimizing temperature-controlled ionic liquid-based microextraction for the determination of histamine residual in canned fish products

摘要

A rapid and simple analytical procedure for the determination of histamine in canned fish products using temperature-controlled ionic liquid-based microextraction (TC-IL-ME) mated to chemometric tool and coupled to spectrophotometer was developed. The variables of interest in the TC-IL-ME method, such as volume of disperser solvent, pH, tartarazine (as ligand) amount, extraction temperature as well as ionic liquid amount were optimized by using a response surface methodology (RSM) based on central composite design (CCD). The ionic liquid, 1-hexyl-3- methylimidazolium tetrafluoroborate [Hmim] [BF4] was used as an extraction solvent, while acetone was used as a disperser solvent. The optimum TC-IL-ME conditions were found as pH 6.0 of sample solution, 60.7 mu mol L(-1 )of tartarazine amount, 765 mu L of acetone volume, 0.5 mg of [Hmim] [BF4] amount and 36 degrees C of extraction temperature. Interactions between tartrazine and histamine were also investigated using computational chemistry approach. Under the selected conditions, the calibration curve was linear in the range of 2-400 ng mL(-1). The repeatability and reproducibility (RSD%) were ranged between 2.5-3.7 % and 2.8-4.1 %, respectively and limit of detection (LOD) was calculated to be 0.65 ng mL(-1). The accuracy of the recommended method was confirmed by the analysis of reference material (TETO4ORM-Histamine in canned fish), the spike test showed recoveries between 92.4 % and 104.9 %. Finally, the recommended method was successfully applied to canned fish products, the concentration of histamine ranged from 62.8-287.5 mu g.g(-1).