Day and blue light modify growth, cell physiology and indole-3-acetic acid production ofAzospirillum brasilenseAz39 under planktonic growth conditions

摘要

Aim In this work, we evaluated the effects of light on growth, cell physiology and stress response ofAzospirillum brasilenseAz39, a non-photosynthetic rhizobacteria, under planktonic growth conditions. Methods and results Exponential cultures of Az39 were exposed to blue (BL), red (RL) and daylight (DL) or maintained in darkness for 24, 48 and 72 h. The biomass production and indole 3-acetic acid (IAA) biosynthesis increased by exposition to DL. Conversely, BL decreased IAA concentration through a direct effect on the molecule. The DL increased superoxide dismutase activity, hydrogen peroxide and thiobarbituric acid reactive substances levels, but the last one was also increased by BL. Both DL and BL increased cell aggregation but only BL increased biofilm formation. Conclusions We demonstrated that both BL and DL are stress effectors forA. brasilenseAz39 under planktonic growth conditions. The DL increased biomass production, IAA biosynthesis and bacterial response to stress, whereas BL induced cell aggregation and biofilms formation, but decreased the IAA concentration by photooxidation. Significance and Impact of the Study Blue light and DL changes growth capacity, cell physiology and plant growth promotion ability ofA. brasilenseAz39 and these changes could be considered to improve the production and functionality of biofertilizers.