Design of a photocleavable drug binding platform for a novel remotely controllable drug coated balloon

摘要

Drug coated balloons have been emerging as alternatives to conventional devices such as drug eluting stents in the treatment of coronary artery stenosis. However, no coating method has been developed to deliver sufficient drug to the lesion. To enhance delivery efficiency- both by reducing the drug wash off under bloodstream and increasing transfer from balloon surface to the arterial wall, we have proposed a photocleavable drug binding platform, which securely bonds the drugs onto the balloon surface, and releases at the site of stenosis triggered by photoirradiation. As a proof of concept, we designed a model system by conjugating 3-amino-3-(2-nitro-phenyl) propionic acid (ANP) onto Cy5, the model drug, then conjugating this onto latex beads, which has a formulation similar to the materials used for conventional balloons. We confirmed its photoresponsivity and release efficiency by irradiating 365 nm light to trigger release and evaluated cellular uptake of Cy5 in vitro. Results proved that the proposed platform releases Cy5 when, and only when, it is triggered by photoirradiation, with drug wash off rate as low as 11% of the conventional DCB coatings in blood-filled environment. Our in vitro evaluation showed that the released substance remains functional and enters cells as designed. The proposed concept may provide a method of highly efficient remotely controllable topical drug delivery through various lumen-like tissues.