Simultaneous determination of olaquindox, oxytetracycline and chlorotetracycline in feeds by high performance liquid chromatography with ultraviolet and fluorescence detection adopting online synchronous derivation and separation

摘要

Olaquindox, oxytetracycline and chlorotetracycline were widely used in feed as antibiotics and growth promoter to improve feed conversion efficiency and increase the rate of weight gain for animals. However, the use of these antibiotics in feed was gradually prohibited because of concerns about contamination and resistance in animals. A quantitative and confirmatory method for determining the presence of olaquindox, oxytetracycline and chlorotetracycline in feed by high performance liquid chromatography equipped with ultraviolet detector in series with fluorescence detector (HPLC-UVD-FLD) was developed, optimized, and validated in three different matrices (compound, concentrated and premix feed). The analytes extraction was performed with a mixture of acetonitrile and 0.1 mol/L ethylenediamine tetraacetic acid disodium-Mcllvaine buffer (1:4, v/v) by one step sample preparation procedure. The validated method presented a broad linear range and good linearity with weighted least square method. The decision limit of the analytes ranged from 0.61 to 0.77 mg/kg for olaquindox, 0.90 to 1.2 mg/kg for oxytetracycline and 1.3 to 2.0 mg/kg for chlorotetracycline. The average recovery values found in intermediate precision conditions were ranged from 88.0 to 99.7% for olaquindox with RSD lower than 11.1%, from 84.4 to 99.0% for oxytetracycline with RSD lower than 9.6%, from 83.8 to 97.5% for chlorotetracycline with RSD lower than 10.0%. By Youden test and bottom-up method, the method was proved to be sufficiently robust and had a small uncertainty for different concentration levels. The developed method was successfully utilized for commercial feed samples to monitor complex cross contamination and residue conditions. Online synchronous derivation and separation using ultraviolet detector in series with fluorescence detector can effectively prevent false positive of chlorotetracycline in feed caused by vegetable meal. Since olaquindox, oxytetracycline and chlorotetracycline ar