Mechanism of Dandelion Sterol in Treating Pulmonary Fibrosis Through Transforming Growth Factor-beta Signaling Pathway

摘要

Background: The paper aimed to elucidate the molecular mechanism of Dandelion sterol in the treatment of pulmonary fibrosis, to study its effect on EMT of lung epithelial cells, and to find its target and downstream signaling pathways. Material and methods: The effects of Dandelion sterol on parathyroid (PQ)-induced EMT in lung epithelial cells were studied by immunofluorescence method. Immunohistochemistry and western-blot methods were used to verify that Dandelion sterol inhibited TGF-beta 1-induced EMT at the cellular level in animals, demonstrating that Dandelion sterol targets TGF-beta 1 to exert an anti-pulmonary fibrosis effect. Results: Dandelion sterol significantly inhibited PQ-induced migration and invasion of lung epithelial cells, and also inhibited the induced EMT. Dandelion sterol had a proper binding activity with the lung fibrosis-inducing factor TGF-beta 1. Dandelion sterol inhibited the TGF-beta 1-induced EMT process, and acted to treat pulmonary fibrosis by inhibiting the TGF-beta 1/Smad3 signaling pathway. Conclusion: Dandelion sterol can inhibit the pulmonary fibrosis by inhibiting the EMT process of lung epithelial cells through targeting the TGF-beta 1/Smad signaling pathway.