Development of Herbal Antibiotic Formulation against Staphylococcus aureus from leaf extract of Datura inoxia containing Triacylglycerol

摘要

Recent emergence of antibiotic resistance and related toxicity issues limit the use of currently available antibiotics. This problem has forced many scientists to search for alternative drug line that would combat resistant pathogens. The use of plants as natural substitute is the paramount area of research to overcome the drug resistance problems, due to their comparable safety and efficacy. Considering these observations, we have studied the effect of hexane extract of Datura inoxia leaf against Staphylococcus aureus. The extract was checked for its antibacterial activity in both crude and purified state. The most effective fraction was further analyzed by HPLC, ESI-MS and bioinformatics tool for compound identification. The spectrum of the unknown component was compared with the spectrum of the known components stored in the NIST library. Furthermore, herbal formulation (gel) from the most effective purified fraction was also prepared and found to give good antibacterial effect. The name, molecular weight, and structure of the components of the test material were ascertained and the compound was identified as Triacylglycerol. Herbal formulation (gel) also showed significant result. Present study provides a good support for the therapeutic applicability of Triacylglycerol. To further confirm the effectiveness of compound, its mechanistic, cytotoxicity and in vivo studies are required.