Protective Effects of Zinc Supplement on Chromatin Deficiency and Sperm Parameters in Streptozotocin-Induced Diabetic Rats

摘要

Objective: Diabetes mellitus (DM) is one of the metabolic diseases that may effect on sperm's health and male reproductive function. Also zinc supplement had many antioxidant agents that may improve adverse effects of diabetes on DNA integrity and sperm chromatins. Materials and methods: In this study 36 Wistar Rat (200 gr) were randomly divided non-diabetic and Streptozotocin-induced diabetic, then were divided into 4 groups (n = 9): zinc sulfate (25 mg daily) and sailin administration. Then sperm parameters and semen analysis were assessed, thirty-five days later according to World Health Organization (WHO) standards. The sperm DNA fragmentation was measured by sperm chromatin dispersion (SCD) test, protamine deficiency was determined by chromo-mycin A3 (CMA3) staining. Results: The sperm count and viability increased in zinc supplement groups, also head and neck deformity were seen less than saline administration groups(p < 0.05). In Zinc administration groups, the sperm motility type C, D reduced; furthermore abnormal morphology, DNA fragmentation and protamine deficiency over than 30% were reduced (p < 0.05). Conclusion: The results show the protective effects of Zinc supplement on sperm parameters, chromatin efficiency and DNA integrity caused by diabetes. The beneficial effect of Zinc supplement might be associated to the inhibition of oxidative stress accordingly a favorable antioxidant therapeutic candidate for the management of male infertility.