EFFECT OF COCONUT WATER IN TRIS EGG YOLK CITRATE EXTENDER ON CAUDA EPIDIDYMAL BUCK SPERMATOZOA MOTILITY PRESERVED AT REFRIGERATION TEMPERATURE

摘要

The present research work was carried out on total ten (10) pairs of testis from ten (10) matured non-descript buck irrespective of breed presented for slaughter at government approved slaughter house, over a period of four months from August to November, 2018. The objective of present study was to evaluate the effect of coconut water supplementation in tris egg yolk citrate extender on cauda epididymal buck spermatozoa motility preserved at refrigeration temperature. The paired cauda epididymal spermatozoa were diluted with tris egg yolk citrate extender and made a five equal aliquots i.e. T1 to T5. T1 is kept as a control while T2, T3, T4 and T5 were supplemented with 5, 10, 15 and 20% coconut water and preserved at refrigeration temperature (4-5degreeC). The sperm motility and motility degeneration rate (MDR) were measured at different time intervals up to 48 hrs. The highest percentage of sperm motility, irrespective of preservation time, was found in T2 (59.80±3.49 %) followed by T3 (53.40±3.83 %), T4 (46.35±4.19 %), T1 (43.05±4.53 %) and T5 (42.30±4.35 %) groups in paired cauda epididymis buck spermatozoa at refrigeration temperature. Further, the percentage of sperm motility was decreased with increased preservation time. The least percentage of motility degeneration rate, irrespective of preservation time, was found in T2 (24.03±3.74 %) followed by T3 (34.92±3.79 %), T4 (47.03±3.93 %), T1 (51.59±4.41 %) and T5 (53.16±3.86 %) groups in paired cauda epididymal buck spermatozoa atrefrigerated temperature, respectively.