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首页> 外文期刊>Biotechnology and Bioengineering >Rational modification of Corynebacterium glutamicumCorynebacterium glutamicum dihydrodipicolinate reductase to switch the nucleotide‐cofactor specificity for increasing ll ‐lysine production
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Rational modification of Corynebacterium glutamicumCorynebacterium glutamicum dihydrodipicolinate reductase to switch the nucleotide‐cofactor specificity for increasing ll ‐lysine production

机译：椰子杆菌谷氨酰胺谷氨酰胺二水二巯基氨基吡啶膦酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸盐酸酯的补充剂的增加l< /sc -lysine生产

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Abstract <section xml:id="bit26591-sec-0010" numbered="no"> > <sc>l</sc> ‐lysine is an important amino acid in animals and humans and NADPH is a vital cofactor for maximizing the efficiency of <sc>l</sc> ‐lysine fermentation. Dihydrodipicolinate reductase (DHDPR), an NAD(P)H‐dependent enzyme, shows a variance in nucleotide‐cofactor affinity in bacteria. In this study, we rationally engineered Corynebacterium glutamicum DHDPR ( Cg DHDPR) to switch its nucleotide‐cofactor specificity resulting in an increase in final titer (from 82.6 to 117.3?g?L ?1 ), carbon yield (from 0.35 to 0.44?g [g glucose] ?1 ) and productivity (from 2.07 to 2.93?g?L ?1 ?hr ?1 ) of <sc>l</sc> ‐lysine in JL‐6 Δ dapB::Ec ‐ dapB C115G,G116C in fed‐batch fermentation. To do this, we comparatively analyzed the characteristics of Cg DHDPR and Escherichia coli DHDPR ( Ec DHDPR), indicating that hetero‐expression of NADH‐dependent Ec DHDPR increased <sc>l</sc> ‐lysine production. Subsequently, we rationally modified the conserved structure of cofactor‐binding motif, and results indicated that introducing the mutation K11A or R13A in Cg DHDPR and introducing the mutation R16A or R39A in Ec DHDPR modifies the nucleotide‐cofactor affinity of DHDPR. Lastly, the effects of these mutated DHDPRs on <sc>l</sc> ‐lysine </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“bit26591-sec-0010”编号=“否”> > <sc> l <sc> -lysine是动物中的重要氨基酸，人类和nadph是最大化<sc>的效率的重要辅助因子l </ sc> -lysine发酵。二水解胆碱酸还原酶（DHDPR），NAD（P）H依赖性酶显示出细菌中核苷酸 - 辅因子亲和力的差异。在这项研究中，我们理性地设计了棒状杆菌（Corynebacterium）</ i> DHDPR（ CG </ i> DHDPR），以改变其核苷酸 - 辅因子特异性，导致最终滴度的增加（从82.6到117.3？g？ l 1 </ sup>），碳产率（从0.35至0.44Ω·g [g葡萄糖] Δ1）和生产率（从2.07到2.93？g？l ？ <sc> l </ sc> -lysine的1 </ sup>Δhrα1</ sup>）在JL-6δ DAPB :: EC </ I> - DAPB < / i> c115g，g116c </ sup>在膳食批量发酵中。为此，我们相比分析了 Cg </ i> DHDPR和大肠杆菌（ eC </ i> DHDPR）的特征，表明NADH-的异质表达依赖于 EC </ i> DHDPR增加了<SC> L </ SC> -Lysine生产。随后，我们合理地修改了辅因子结合基序的保守结构，并且结果表明在 CG / DHDPR中引入突变K11a或R13a，并在 EC 中引入突变R16a或R39a DHDPR修饰DHDPR的核苷酸 - 辅因子亲和力。最后，这些突变的DHDPRS对<sc> l </ sc> -lysine的影响 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15017/'>《Biotechnology and Bioengineering》</a> <b style="margin: 0 2px;">|</b><span>2018年第7期</span><b style="margin: 0 2px;">|</b><span>共14页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xu Jian‐Zhong&option=202" target="_blank" rel="nofollow">Xu Jian‐Zhong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Han‐Kun&option=202" target="_blank" rel="nofollow">Yang Han‐Kun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Li‐Ming&option=202" target="_blank" rel="nofollow">Liu Li‐Ming;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Ying‐Yu&option=202" target="_blank" rel="nofollow">Wang Ying‐Yu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Wei‐Guo&option=202" target="_blank" rel="nofollow">Zhang Wei‐Guo;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>The Key Laboratory of Industrial BiotechnologyMinistry of Education School of Biotechnology Jiangnan UniversityWuXi People's Republic of China;</p> <p>The Key Laboratory of Industrial BiotechnologyMinistry of Education School of Biotechnology Jiangnan UniversityWuXi People's Republic of China;</p> <p>State Key Laboratory of Food Science and Technology School of BiotechnologyJiangnan UniversityWuXi People's Republic of China;</p> <p>The Key Laboratory of Industrial BiotechnologyMinistry of Education School of Biotechnology Jiangnan UniversityWuXi People's Republic of China;</p> <p>The Key Laboratory of Industrial BiotechnologyMinistry of Education School of Biotechnology Jiangnan UniversityWuXi People's Republic of China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Corynebacterium glutamicum&option=203" rel="nofollow">Corynebacterium glutamicum;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=dihydrodipicolinate reductase&option=203" rel="nofollow">dihydrodipicolinate reductase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=l ‐lysine production&option=203" rel="nofollow">l ‐lysine production;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=nucleotide‐cofactor specificity&option=203" rel="nofollow">nucleotide‐cofactor specificity;</a> </p> <div class="translation"> 机译：棒状杆菌谷氨酸;二氢纤维素酸盐还原酶;L-碘碱生产;核苷酸 - 辅因子特异性; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021882120.html">Rational modification of <i xmlns="http://www.wiley.com/namespaces/wiley">Corynebacterium glutamicumCorynebacterium glutamicum dihydrodipicolinate reductase to switch the nucleotide‐cofactor specificity for increasing <sc xmlns="http://www.wiley.com/namespaces/wiley">l</sc>l ‐lysine production</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xu Jian‐Zhong&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Xu Jian‐Zhong,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Han‐Kun&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yang Han‐Kun,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Li‐Ming&option=202" target="_blank" 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