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Method of thickness measurement for transparent specimens with chromatic confocal microscopy

机译:透明标本厚度测量方法,具有彩色共焦显微镜

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摘要

In this paper, a new method for measuring the thickness of transparent specimens using chromatic confocal microscopy (CCM) is presented. The conventional CCM thickness measurement model relies on capturing the focal points on the upper and lower surfaces of a transparent specimen. This model has strict specimen placement tolerance and a limited measurement range. In order to overcome these limitations, a new thickness measurement model was developed by adding an auxiliary reflector below the specimen. The thickness of the specimen can be determined by comparing the wavelengths of light focused on the auxiliary reflector before and after placing the measurement specimen. Theoretical analysis and simulation showed that the proposed method has twice the measurement range of the conventional model. In order to verify the proposed CCM measurement model, a laboratory thickness measurement system was developed by the authors' team. A commercial laser scanning confocal microscope (Carl Zeiss LSM780) was used as the reference system. A set of quartz glasses was measured using both the proposed system and the reference system. Experimental comparison showed that the proposed method was able to achieve a measurement accuracy of 0.25 mu m. In addition, repeated measurements conducted at different heights showed negligible variation. Thus, it can be concluded that the specimen placement tolerance was improved significantly compared with the conventional model. (C) 2018 Optical Society of America
机译:本文介绍了一种用于测量使用核共聚焦显微镜(CCM)的透明试样厚度的新方法。传统的CCM厚度测量模型依赖于捕获透明样品的上表面和下表面上的焦点。该模型具有严格的标本放置容差和有限的测量范围。为了克服这些限制,通过在样本下方添加辅助反射器来开发新的厚度测量模型。可以通过将聚焦在辅助反射器上的光的波长与放置测量样本之前和之后进行比较来确定样本的厚度。理论分析和仿真表明,所提出的方法具有传统模型的测量范围的两倍。为了验证所提出的CCM测量模型,作者团队开发了实验室厚度测量系统。商业激光扫描共聚焦显微镜(Carl Zeiss LSM780)用作参考系统。使用所提出的系统和参考系统测量一组石英玻璃。实验比较表明,该方法能够达到0.25μm的测量精度。另外,在不同高度下进行的重复测量显示出可忽略的变化。因此,可以得出结论,与传统模型相比,样本放置耐受性显着提高。 (c)2018年光学学会

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  • 来源
    《Applied optics》 |2018年第33期|共7页
  • 作者单位

    Huaqiao Univ Coll Mech Engn &

    Automat Xiamen 361021 Peoples R China;

    Huaqiao Univ Coll Mech Engn &

    Automat Xiamen 361021 Peoples R China;

    Huaqiao Univ Inst Mfg Technol Xiamen 361021 Peoples R China;

    Huaqiao Univ Coll Mech Engn &

    Automat Xiamen 361021 Peoples R China;

    Huaqiao Univ Coll Mech Engn &

    Automat Xiamen 361021 Peoples R China;

    Huaqiao Univ Coll Mech Engn &

    Automat Xiamen 361021 Peoples R China;

    Huaqiao Univ Coll Mech Engn &

    Automat Xiamen 361021 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 应用;
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